About InVivoMAb anti-mouse CXCR2 (CD182) The Cx2Mab-1 antibody reacts with mouse CXC chemokine receptor 2 (CXCR2), also known as CD182, GRO/MGSA receptor, and IL-8RB. CXCR2 is a G protein-coupled receptor (GPCR) that coordinates immune cell trafficking during immune responses. CXCR2 ligands include its primary ligands CXCL8 (IL-8) and CXCL1 (GRO-α) as well as CXCR2, CXCL2, CXCL3, CXCL5, CXCL6, and CXCL7. Leukocytes (particularly neutrophils and monocytes) constitutively express CXCR2, whereas parenchymal cells such as fibroblasts, hepatocytes, and neurons also express this receptor to varying degrees of abundance. CXCR2 and its ligands facilitate various tissue-dependent signals for regulating cellular survival, proliferation, differentiation, adhesion, and migration. In cancer tissues, CXCR2 signaling is critical to the processes of tumor angiogenesis, growth, and chemoresistance, and a high expression of CXCR2 on myeloid cells drives their migration to the tumor cells. Blockade of CXCR2-mediated recruitment of myeloid-derived suppressor cells (MDSCs) into the tumor microenvironment and the pre-metastatic niche is emerging as an attractive approach to boost cancer immunotherapy. The Cx2Mab-1 antibody targets the N-terminal extracellular domain of CXCR2, which is important for ligand binding and receptor activation. This antibody could be a useful tool for CXCR2 blockade in both in vitro and in vivo preclinical experimental studies. InVivoMAb anti-mouse CXCR2 (CD182) Specifications IsotypeRat IgG2b, κ Recommended Isotype Control(s)InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenSynthetic peptide corresponding to mouse CXCR2’s N-terminal sequence - MGEFKVDKFNIEDFFSGDL Reported ApplicationsFlow cytometry For details on in vivo applications, please contact technicalservice@bioxcell.com FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtered ProductionPurified from cell culture supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
