About InVivoMAb anti-mouse CXCR4 (CD184) The Cx4Mab-1 monoclonal antibody reacts with mouse CXC chemokine receptor 4 (CXCR4), also called fusin and CD184. CXCR4 is a hepta-transmembrane domain G protein-coupled receptor (GPCR) that is expressed in hematopoietic cells, endothelial cells, neurons, and embryonic as well as adult stem cells. The primary ligand of CXCR4 is CXCL12, also known as stromal cell-derived factor-1α (SDF-1α). The CXCR4-CXCL12 signaling pathway plays a critical role in activating multiple signaling pathways including ERK1/2, ras, p38 MAPK, PLC/MAPK, and SAPK/JNK. CXCR4-CXCL12 signaling also regulates several biological processes including cell survival, proliferation, migration, and stemness. Overexpression of CXCR4 is often linked to poor prognosis in various cancers, and during cancer metastasis, CXCR4 positive cancer cells are chemotactically homed to tissues such as the liver, bone marrow, lungs, and lymph nodes that express abundant levels of CXCL12. CXCR4 as well as CXCR4/CXCL12 antagonists are emerging as attractive targets for experimental cancer therapeutics. The anti-mouse CXCR4 antibody, clone Cx4Mab-1, was generated using a cell-based immunization and screening approach. Its high affinity for both endogenous and exogenous mouse CXCR4 suggests its potential utility in in vivo preclinical mouse models. InVivoMAb anti-mouse CXCR4 (CD184) Specifications IsotypeRat IgG2a, κ Recommended Isotype Control(s)InVivoMAb rat IgG2a isotype control, anti-trinitrophenol Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenmCXCR4-PA tag overexpressing LN229 cells Reported ApplicationsFlow cytometry For details on in vivo applications please contact technicalservice@bioxcell.com FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtered ProductionPurified from cell culture supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze. Application ReferencesInVivoMAb anti-mouse CXCR4 (CD184) (CLONE: Cx4Mab-1)Ouchida T, Suzuki H, Tanaka T, Kaneko MK, Kato Y (2024). "Cx4Mab-1: A Novel Anti-Mouse CXCR4 Monoclonal Antibody for Flow Cytometry" Monoclon Antib Immunodiagn Immunother 43(1):10-16. PubMedThe CXC chemokine receptor 4 (CXCR4, CD184) is a member of the G protein-coupled receptor family that is expressed in most leukocytes. Overexpression of CXCR4 is associated with poor prognosis in not only hematopoietic malignancy but also solid tumors. Because CXCR4 is an attractive target for tumor therapy, reliable preclinical murine models using anti-CXCR4 monoclonal antibodies (mAbs) have been warranted. This study established a novel anti-mouse CXCR4 (mCXCR4) mAb using the Cell-Based Immunization and Screening method. Flow cytometric analysis showed that an anti-mCXCR4 mAb, Cx4Mab-1 (rat IgG2a, kappa), recognized mCXCR4-overexpressed Chinese hamster ovary-K1 (CHO/mCXCR4) cells and endogenously mCXCR4-expressing mouse myeloma P3X63Ag8U.1 (P3U1) cells. Furthermore, Cx4Mab-1 did not recognize mCXCR4-knockout P3U1 cells. The dissociation constants of Cx4Mab-1 for CHO/mCXCR4 and P3U1 were determined as 6.4 × 10-9 M and 2.3 × 10-9 M, respectively, indicating that Cx4Mab-1 possesses a high affinity to both endogenous and exogenous mCXCR4-expressing cells. These results indicate that Cx4Mab-1 could be a useful tool for preclinical mouse models.
