About InVivoMAb anti-mouse/human EGFR (domain III) The EMab-88 monoclonal antibody reacts with domain III of the mouse and human epidermal growth factor receptor (EGFR), also known as ERBB, ERBB1, and HER1. EGFR is a transmembrane glycoprotein and a member of the ErbB family of receptor protein tyrosine kinases (RTKs). The extracellular region of EGFR has four domains, wherein domains I and III contain the ligand-binding site. EGFR ligands include EGF, AREG, TGF-α, epiregulin (EREG), epigen, betacellulin, and heparin-binding EGF. Upon ligand binding, EGFR dimerizes, and its activation through the autophosphorylation of its cytoplasmic domain triggers several downstream signaling pathways, including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC-γ-PKC, and STATs, which help the cell respond accurately to extracellular signals. EGFR is expressed ubiquitously, and in various tissues, it plays critical roles in the developmental, physiological, and regenerative processes. EGFR’s aberrant activation is a common feature of various malignancies. Using monoclonal antibodies to target the EGFR extracellular region has shown promising results in anti-cancer research, and currently approved therapeutic antibodies such as cetuximab, panitumumab, nimotuzumab, and necitumumab bind to domain III of EGFR, which directly blocks EGF-EGFR binding. InVivoMAb anti-mouse/human EGFR (domain III) Specifications IsotypeMouse IgG1, κ Recommended Isotype Control(s)InVivoMAb mouse IgG1 isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenHuman EGFR (ErbB1) Reported ApplicationsFlow cytometry For details on in vivo applications, please contact technicalservice@bioxcell.com FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtered ProductionPurified from cell culture supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.