About InVivoMAb anti-rat L-selectin (CD62L) The OX-85 monoclonal antibody reacts with rat L-selectin (CD62L), also known as LECAM-1, Sell, LAM-1, Ly-22, and Lymphocyte surface MEL-14 antigen. CD62L is a single-pass type I membrane glycoprotein from the selectin family. This protein is expressed by the majority of naïve T and B cells, neutrophils, monocytes, a subset of memory T cells, NK cells, and most thymocytes. CD62L is a calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. CD62L is a cell adhesion molecule (CAM) that binds to many glycoprotein ligands, including CD34, GlyCAM-1, MAdCAM-1, PODXL2, and PSGL-1. CD62L’s interaction with SELPLG/PSGL1 (CD162) and PODXL2 promotes recruitment and rolling of leukocytes. CD62L acts as a “homing receptor” for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Moreover, CD62L facilitates the emigration of neutrophils towards the sites of inflammation. During activation of leukocytes (including lymphocytes and neutrophils), the CD62L protein is enzymatically cleaved from the cell surface by the ADAM17 enzyme. Owing to its role in leukocyte biology, CD62L shedding acts as an excellent marker in cancer, infectious, inflammatory, and autoimmune diseases. Manipulating CD62L shedding is also considered a strategy to enhance the efficacy of immunotherapeutics, e.g., through augmenting the lytic activity of T cells against tumors. InVivoMAb anti-rat L-selectin (CD62L) Specifications IsotypeMouse IgG1, κ Recommended Isotype Control(s)InVivoMAb mouse IgG1 isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenRat purified CD62L-CD4 Reported Applicationsin vivo blocking of L-selectin (CD62L) in vitro blocking of L-selectin (CD62L) in vitro functional assay Flow cytometry ELISA FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin≤1EU/mg (≤0.001EU/μg)Determined by LAL gel clotting assay Purity≥95% Determined by SDS-PAGE Sterility0.2 μm filtered ProductionPurified from cell culture supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze. Application ReferencesInVivoMAb anti-rat L-selectin (CD62L) (CLONE: OX-85)Milićević NM, Nohroudi K, Milićević Z, Westermann J (2004). "Blood lymphocytes, monocytes and NK cells modulate their expression of CD44, ICAM-1, LFA-1 and MHC class II after arrival into lymphoid organs" Immunol Invest 33(4):439-52. PubMedAdhesion molecules expressed on surface membranes of lymphocytes and other leukocytes enable their entry into the lymphoid and other tissues. However, little is known about molecules that govern the transit of leukocytes through the parenchyma of lymphoid organs proper. We show that in comparison to blood leukocytes, the corresponding cells isolated from lymphoid organs, i.e., lymph nodes and spleen, have a significantly augmented expression of certain surface molecules. The helper and cytotoxic subsets of T cells, as well as B cells, display the increased expression of CD44, ICAM-1 and LFA-1, whereas B cells additionally show the augmented expression of MHC class II. In comparison with blood monocytes, splenic monocytes show the increased expression of ICAM-1 and MHC class II molecules. When compared with blood NK cells, splenic NK cells only show the increased expression of ICAM-1. The molecules, which we show to be up regulated upon the entry of leukocytes into lymphoid organs, could be involved in their retention within the tissue via cell-cell or cell-extracellular matrix interactions and in control of their transit through lymphoid tissues.
