About InVivoSIM anti-human CD27 (Varlilumab Biosimilar) This non‑therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Varlilumab, making it ideal for research use. Varlilumab is a human IgG1 monoclonal antibody that specifically targets CD27, a member of the tumor necrosis factor receptor (TNFR) superfamily. CD27 is a co-stimulatory immune receptor that is constitutively expressed on naive and memory T cells, and can also be found on regulatory T cells (Tregs), NK cells, and some B cells. Engagement of CD27 by its ligand, CD70, promotes T cell activation, survival, and effector function, playing a key role in regulating immune responses and maintaining long-term immunity. Varlilumab is an agonist antibody that mimics CD70 binding, delivering potent co-stimulatory signals to T cells while also depleting CD27⁺ regulatory T cells via Fc-mediated effector mechanisms. Varlilumab has shown therapeutic potential in enhancing anti-tumor immunity in cancer models and has been investigated in combination with immune checkpoint inhibitors such as anti-PD-1. This Varlilumab biosimilar is ideal for studying CD27 signaling, T cell costimulation, Treg modulation, and combination immunotherapy mechanisms in both in vitro and in vivo settings. InVivoSIM anti-human CD27 (Varlilumab Biosimilar) Specifications IsotypeHuman IgG4, κ Recommended Isotype Control(s)RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenHuman CD27 Reported ApplicationsIn vivo functional assays in vitro functional assays Flow cytometry Western blot ELISA FormulationpH 7.0 Contains no stabilizers or preservatives Endotoxin≤0.5EU/mg (≤0.0005EU/μg)Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity≥95%Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from cell culture supernatant in an animal-free facility PurificationProtein A Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
