About InVivoSIM anti-human CEACAM5 (Labetuzumab Biosimilar) This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Labetuzumab, making it ideal for research use. This Labetuzumab biosimilar is a humanized anti-carcinoembryonic antigen antibody, and it reacts with carcinoembryonic antigen-related cell adhesion molecule 5 (i.e., CEACAM5), which is also called CEA or CD66e. During fetal or early embryonic stages, CEACAM5 is expressed in the digestive system, and it is found over-expressed in cancerous tissues. CEACAM5 acts as a cell adhesion molecule (CAM), and it inhibits anoikis, a type of apoptosis that is induced upon cell detachment from the ECM. Through this mechanism, CEACAM5 facilitates tumorigenesis and metastasis. CEACAM5 expression is often found upregulated in a variety of human epithelial malignancies, such as breast cancer, colon cancer, lung cancer, neuro-endocrine prostate cancer (NEPC), and pancreatic cancers. In cancer immunotherapy research, CEACAM5-targeted vaccines, bispecific T cell engagers (BiTEs), CAR T cell therapies, and antibody-drug conjugates are routinely tested in various experimental models. In vitro and in vivo studies involving the use of the humanized anti-carcinoembryonic antigen antibody Labetuzumab have shown the antibody to inhibit the cell proliferation and growth of CEACAM5+ tumors. InVivoSIM anti-human CEACAM5 (Labetuzumab Biosimilar) Specifications IsotypeHuman IgG1, κ Recommended Isotype Control(s)RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenHuman CEACAM5 Reported Applicationsin vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from cell culture supernatant in an animal-free facility Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
