About InVivoSIM anti-human IL-6 (Siltuximab Biosimilar)
This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Siltuximab making it ideal for research use. This Siltuximab biosimilar reacts human IL-6 (interleukin-6) a 21-28 kDa cytokine that is expressed by many cell types, including T lymphocytes, B lymphocytes, monocytes, fibroblasts, and endothelial cells. IL-6 signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal-transducing component gp130 (also called CD130). Upon receptor binding IL-6 influences antigen-specific immune responses, inflammatory responses, neuronal development, and is a major mediator of the acute phase reaction. Siltuximab blocks the binding of IL-6 to soluble and membrane bound IL-6R.
InVivoSIM anti-human IL-6 (Siltuximab Biosimilar) Specifications
| Isotype | Human IgG1, κ |
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Human IL-6 |
| Reported Applications | Functional assays ELISA |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| Aggregation | <5% Determined by SEC |
| Purity | >95% Determined by SDS-PAGE |
| Sterility | 0.2 μm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |