About InVivoSIM anti-human mesothelin (Amatuximab Biosimilar) This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Amatuximab, making it ideal for research use. Amatuximab is a high-affinity chimeric monoclonal antibody that reacts with mesothelin (MSLN). Human MSLN is synthesized as a 71-kDa precursor that translocates to the cell surface, where it is cleaved into 31-kDa megakaryocyte potentiating factor (MPF) and 40-kDa mature MSLN protein. MSLN is a glycosylphosphatidyl inositol (GPI)-anchored membrane glycoprotein whose expression in adult humans is restricted to mesothelium. Notably, MSLN is found over-expressed in many epithelial cancers, such as epithelial mesotheliomas, ovarian cancers, lung adenocarcinomas, and pancreatic ductal adenocarcinomas. MSLN interacts with cancer antigen 125 (CA125), a specific epitope expressed on MUC16, and it is suggested to play a role in tumorigenesis and metastasis. The tumor-specific expression of MSLN makes it an attractive target antigen for cancer immunotherapy. Amatuximab has been shown to elicit antibody-dependent cellular cytotoxicity (ADCC) against MSLN+ cancer cell lines in vitro, and a greater efficacy in mouse tumor xenografts when combined with chemotherapy. InVivoSIM anti-human mesothelin (Amatuximab Biosimilar) Specifications IsotypeHuman IgG1, κ Recommended Isotype Control(s)RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenHuman mesothelin Reported Applicationsin vivo functional assays in vitro functional assays ELISA Western blot Flow cytometry FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from cell culture supernatant in an animal-free facility Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
