About InVivoSIM anti-human TSLP (Tezepelumab Biosimilar) This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Tezepelumab making it ideal for research use. The Tezepelumab biosimilar antibody reacts with human Thymic Stromal-Derived Lymphopoetin (TSLP). TSLP is a cytokine that is closely related to IL-7. TSLP is expressed predominantly by epithelial cells in the thymus, lung, skin, intestine, and tonsils, as well as stromal cells, mast cells, and dendritic cells. TSLP exerts its biological activities by binding to a heterodimeric receptor that consists of IL-7Rα and the TSLP receptor (TSLPR) chain. Expression of TSLP is regulated by NF-κB and can be induced by exposure to viral, bacterial, or parasitic products, inflammatory cytokines, and the Th2 cell–associated cytokines IL-4 and IL-13. TSLP has important roles in conditioning DCs to drive Th2 differentiation and its expression is increased in immunopathologies associated with dysregulated Th2 cell–type cytokine production, including atopic dermatitis and asthma. It has additionally been reported to promote antibody isotype class switching in B cells, to regulate the development of Foxp3+ Tregs in the human thymus, and to promote basophil hematopoiesis. Tezepelumab was developed for treating moderate-to-severe asthma by specifically binding to TSLP and preventing its binding to the TSLP receptor on inflammatory cells. InVivoSIM anti-human TSLP (Tezepelumab Biosimilar) Specifications IsotypeHuman IgG2, λ Recommended Isotype Control(s)InVivoPlus human IgG2 isotype control Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenHuman TSLP Reported ApplicationsFunctional assays FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from cell culture supernatant in an animal-free facility PurificationProtein A Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
