About RecombiMAb anti-mouse 4-1BBL (CD137L) (D265A) The TKS-1-CP039 monoclonal antibody is a chimeric version of the original TKS-1 antibody. The variable domain sequences are identical to the original TKS-1 but the constant region sequences have been switched from rat IgG2a to mouse IgG1. The TKS-1-CP039 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The TKS-1-CP039 monoclonal antibody reacts with mouse 4-1BB ligand (4-1BBL), a type II transmembrane glycoprotein also known as CD137L. 4-1BBL is a 97 kDa member of the TNF superfamily and is expressed by dendritic cells, macrophages, and activated B and T lymphocytes. Interaction of 4-1BBL with 4-1BB (CD137) provides costimulatory signals to both CD4 and CD8 T cells through the activation of NF-κB, c-Jun and p38 downstream pathways. The TKS-1 antibody has been shown to inhibit the binding of soluble 4-1BB to 4-1BBL in vitro. RecombiMAb anti-mouse 4-1BBL (CD137L) (D265A) Specifications IsotypeMouse IgG1, κ Recommended Isotype Control(s)RecombiMAb mouse IgG1 (D265A) isotype control, anti-hen egg lysozyme Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenMouse 4-1BBL transfected NRK cells Reported Applicationsin vivo 4-1BBL blockade* ELISA* *Reported for the original rat IgG2a TKS-1 antibody FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from HEK293 cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
