About RecombiMAb anti-mouse CD106 (VCAM-1) The M/K-2.7-CP050 monoclonal antibody is a chimeric version of the original M/K-2.7 antibody. The variable domain sequences are identical to the original M/K-2.7 but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The M/K-2.7-CP050 monoclonal antibody reacts with mouse CD106 also known as VCAM-1 and INCAM-110. CD106 is a 110 kDa single chain type I glycoprotein that is expressed primarily on activated vascular endothelial cells but has also been reported on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. CD106 expression is induced by inflammatory stimuli and cytokines. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 which contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation. RecombiMAb anti-mouse CD106 (VCAM-1) Specifications IsotypeMouse IgG2a, κ Recommended Isotype Control(s)InVivoPlus mouse IgG2a isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenStromal cells from mouse bone marrow Reported Applicationsin vivo VCAM-1 neutralization* Immunofluorescence* *Reported for the original rat IgG1 M/K-2.7 antibody FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from HEK293 cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
