About RecombiMAb anti-mouse CD38 The NIMR5-CP052 monoclonal antibody is a chimeric version of the original NIMR5 antibody. The variable domain sequences are identical to the original NIMR5 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The NIMR5-CP052 monoclonal antibody reacts with mouse CD38 a 42 kDa type II transmembrane glycoprotein. CD38 is expressed on immature and mature resting and activated B cells as well as NK cells and a subset of T cells. CD38 expression is down-regulated on mouse germinal center B cells and plasma cells. CD38 is a multifunctional protein involved in NAD homeostasis and in the generation of the second messengers ADPR and cyclic ADPR which are involved in intracellular calcium signaling. Antibodies against CD38 have been shown to be highly efficacious in the treatment of multiple myeloma. The original NIMR5 antibody is reported as an agonistic antibody and has been shown to induce B cell proliferation, protect B cells from apoptosis, and slow disease progression in tumor-bearing mice. RecombiMAb anti-mouse CD38 Specifications IsotypeMouse IgG2a, κ Recommended Isotype Control(s)InVivoPlus mouse IgG2a isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenBCL1 plasma membrane glycoproteins Reported Applicationsin vivo CD38 stimulation* in vitro CD38 stimulation* in vitro B cell activation* Immunofluorescence* ELISA* Flow cytometry* *Reported for the original rat IgG2a NIMR5 antibody FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from HEK293 cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
