About RecombiMAb anti-mouse CD4 The GK1.5-CP127 monoclonal antibody is a chimeric version of the original GK1.5 antibody. The variable domain sequences are identical to the original GK1.5 but the constant region sequences have been switched from rat IgG2b to mouse IgG2b. The GK1.5-CP127 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The GK1.5-CP127 monoclonal antibody reacts with the mouse CD4. The CD4 antigen is a 55 kDa cell surface type I membrane glycoprotein belonging to the immunoglobulin superfamily. CD4 acts as a co-receptor which in cooperation with the T cell receptor (TCR) interacts with class II MHC molecules displayed by antigen presenting cells (APC). CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally. RecombiMAb anti-mouse CD4 Specifications IsotypeMouse IgG2b, κ Recommended Isotype Control(s)InVivoPlus mouse IgG2b isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 6.5 Dilution Buffer ImmunogenMouse CTL clone V4 Reported Applicationsin vivo CD4+ T cell depletion*Flow cytometry*Western blot**Reported for the original rat IgG2b GK1.5 antibody FormulationPBS, pH 6.5 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from CHO cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
