About RecombiMAb anti-mouse FAP The CP057 monoclonal antibody is a recombinant, chimeric version of the original 73.3 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Mouse IgG1, κ to Mouse IgG2a, κ. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The 73.3 monoclonal antibody reacts with mouse fibroblast activation protein (FAP), a cell-surface serine protease that acts on various hormones and extracellular matrix components. FAP is expressed during embryonic development, in tissues of healing wounds, and in chronic inflammatory and fibrotic conditions. FAP protein is highly upregulated in cancer-associated fibroblasts (CAFs) in epithelial tumors. CAF overexpression of FAP promotes tumor development and metastasis by influencing extracellular matrix remodeling, intracellular signaling, angiogenesis, epithelial-to-mesenchymal transition, and immunosuppression. The scFv of the 73.3 antibody has been used to construct 73.3-FAP-CAR T cells specific for FAP. Adoptively transferred 73.3-FAP-CAR T cells have been shown to inhibit the growth of multiple syngeneic mouse tumor models. RecombiMAb anti-mouse FAP Specifications IsotypeMouse IgG2a, κ Recommended Isotype Control(s)RecombiMAb mouse IgG2a isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenImmunization of FAP-null mice with FAP-expressing 3T3 cells Reported ApplicationsImmunoprecipitation Flow cytometry Western blot Chimeric Antigen Receptor construction *Reported for the original 73.3 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin≤0.5EU/mg (≤0.0005EU/μg)Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity≥95%Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from HEK293 cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
