About RecombiMAb anti-mouse IFNAR-1 MAR1-5A3-CP056 monoclonal antibody is a recombinant, chimeric version of the original MAR1-5A3 antibody. The variable domain sequences are identical to clone MAR1-5A3, but the constant region has been converted from mouse IgG1 to mouse IgG2a. MAR1-5A3-CP056 also contains Fc silencing mutations rendering it unable to bind to endogenous Fcγ receptors, similar to therapeutic anti-IFNAR-1 antibodies such as Anifrolumab. These mutations prevent Fc-effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. MAR1-5A3-CP056 antibody reacts with mouse IFNAR-1 (IFN alpha/beta receptor subunit 1). IFNAR-1 is co-expressed with IFNAR-2 on most cell types and together these two subunits make up the heterodimeric Type I IFN Receptor complex. Type I IFNs (IFN-α/β) bind to the Type I IFN Receptor complex to induce cellular responses including induction of anti-viral, anti-microbial, anti-tumor, and autoimmune responses as well as to regulate activation, proliferation, and differentiation. The MAR1-5A3 antibody has demonstrated inhibition of Type I IFN receptor signaling in vitro and in vivo. RecombiMAb anti-mouse IFNAR-1 Specifications Recommended Isotype Control(s)RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenExtracellular domain of mouse IFNAR-1 Reported Applicationsin vitro IFNAR-1 blockade* in vivo IFNAR-1 blockade* *Reported for the original mouse IgG1 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from HEK293 cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
