RecombiMAb anti-mouse LPAM-1 (Integrin α4β7)

Clone Catalog # Category
DATK32-CP069 CP069
USD 541 - USD 7325

About RecombiMAb anti-mouse LPAM-1 (Integrin α4β7)

The DATK32-CP069 monoclonal antibody is a recombinant, chimeric version of the original DATK32 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG2a, κ to mouse IgG2a, κ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The DATK32 monoclonal antibody reacts with mouse LPAM-1 also known as integrin alpha 4 beta 7. The 130 kDa integrin β7 chain associates with the 150 kDa integrin α4 (CD49d) chain to form LPAM-1, a member of the Ig superfamily. LPAM-1 is expressed by peripheral lymphocytes, small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds VCAM-1 (CD106), MAdCAM-1, and fibronectin and facilitates lymphocyte adhesion and migration to the intestine and associated lymphoid tissues. The DATK32 antibody has been reported to block LPAM-1-mediated cell adhesion in vivo.

RecombiMAb anti-mouse LPAM-1 (Integrin α4β7) Specifications

IsotypeMouse IgG2a, κ
ImmunogenTK1 cells
Reported Applicationsin vivo Integrin α4β7 neutralization Flow cytometry For information on in vivo applications, please contact technicalservice@bioxcell.com
FormulationPBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation<5% Determined by SEC
Purity>95% Determined by SDS-PAGE
Sterility0.2 μm filtration
ProductionPurified from HEK293 cell supernatant in an animal-free facility
PurificationProtein G
Molecular Weight150 kDa
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.