About RecombiMAb anti-mouse VISTA The 13F3-CP053 monoclonal antibody is a chimeric version of the original 13F3 antibody. The variable domain sequences are identical to the original 13F3 but the constant region sequences have been switched from Armenian hamster IgG to mouse IgG2a. The 13F3-CP053 antibody contains no Fc mutations just as the original hamster IgG antibody does not. The 13F3-CP053 monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5. VISTA is a 309 aa type I transmembrane glycoprotein and a member of the Ig superfamily. VISTA is expressed on naïve and activated T cells, NK cells, macrophages, dendritic cells, and neutrophils. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. Blockade of VISTA with the original 13F3 antibody results in delayed tumor growth in mouse models of melanoma. RecombiMAb anti-mouse VISTA Specifications IsotypeMouse IgG2a, κ Recommended Isotype Control(s)RecombiMAb mouse IgG2a isotype control, anti-hen egg lysozyme Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenEL4 cells overexpressing mouse VISTA-RFP and then boosted with VISTA-Ig fusion protein Reported Applicationsin vivo blocking of VISTA signaling* in vitro blocking of VISTA signaling* Flow cytometry* *Reported for the original Armenian hamster IgG 13F3 antibody FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from HEK293 cell supernatant in an animal-free facility PurificationProtein A Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
