About InVivoSIM anti-human PD-L1 (Avelumab Biosimilar) This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Avelumab making it ideal for research use. This Avelumab biosimilar reacts with human PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell mediated lysis. Avelumab blocks the interaction of PD-L1 with PD-1 and CD80. InVivoSIM anti-human PD-L1 (Avelumab Biosimilar) Specifications IsotypeHuman IgG1, λ Recommended Isotype Control(s)RecombiMAb human IgG1 isotype control, anti-respiratory syncytial virus Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenFull length human PD-L1 Reported ApplicationsFunctional assays ELISA in vitro PD-L1 blockade FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from cell culture supernatant in an animal-free facility PurificationProtein A Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
