How do I request a quote?
- Navigate to the product page of the first product you would like to add to your quote.
- Once on the product page, switch to quote mode by clicking on “Quote” in the upper section of the page.
- Select your desired size and quantity using the “Choose your option” dropdown menu.
- Click the “Add to Quote” tab to add the product to your quote, a confirmation window will appear.
- Click the ‘Keep Shopping” tab if you have more products to add.
- Repeat this process for all remaining items you would like to add to your quote.
- Once you are ready to submit the quote click the red “Quote” button in the upper right corner and click on “Begin Request”. Alternatively, clicking the “View Quote” button in the confirmation window will also bring you to your quote.
- Complete the request form with your name and address information and submit the request.
- A customer service representative will follow up with you within 1 business day via email.
Can I get a free sample of a product?
We do not offer free or trial sized samples for testing purposes.
How do I store my antibodies?
Store your antibodies undiluted at 4°C in the dark.
We do not recommend freezing antibodies (even when aliquoted) as this can result in a loss of activity caused by the freezing/thawing process.
Additionally, diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided.
Proteins, in general, are less susceptible to degradation when stored at higher concentrations.
Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer (just make sure to keep the antibody sterile).
How do I handle my antibodies?
Always try to maintain the sterility of your antibodies to avoid contamination.
Working with the antibodies in a biological safety cabinet and using sterile pipet tips, tubes, syringes, and buffers will greatly reduce the chances of contamination.
Additionally, try to keep the antibodies cold until just prior to administration.
What is the shelf life of Bio X Cell’s products?
Bio X Cell’s products will remain stable for 1 year from the date of receipt if the antibodies are stored under the conditions outlined on our product data sheets.
How are Bio X Cell’s antibodies supplied?
Our antibodies are supplied in sterile PBS buffer. The solution contains no stabilizers, preservatives, carrier proteins, glycerol, or any other additives.
The solution is simply the antibody in PBS buffer. The exact composition of the PBS varies but the constituents are always Na2HPO4, NaH2PO4, and NaCl.
The pH of the solution will vary depending on the pI of the antibody, this dictates the molarity of each buffer constituent.
In some circumstances we can supply antibodies in other buffers when requested but we are not able to provide antibodies as dry lyophilized powder.
What are the concentrations of Bio X Cell’s antibodies?
The concentration of our products varies from lot to lot but is typically between 4 and 10 mg/ml.
The exact concentration will be marked on the tube itself.
If you need to know the exact concentration of a product before ordering, please contact email@example.com
What is the AA or DNA sequence of the antibody?
Our antibodies are produced using standard hybridoma technology and so the AA or DNA sequence of the antibody is unknown.
What is the epitope of the antibody?
We have not epitope mapped our antibodies at Bio X Cell. Searching the published literature would be the best way to determine if this information is available.
How do I dilute my antibody to a working concentration?
We recommend diluting our antibodies in the InVivoPure dilution buffer matching the pH that the antibody is supplied in. In the absence of InVivoPure dilution buffer sterile 1x PBS matching the exact pH of the antibody can be used. It is important to match the pH of the dilution buffer to the antibody to avoid aggregation.
When diluting we recommend using cold buffer and maintaining sterility by working in a biological safety cabinet and using sterile pipet tips, tubes, syringes, and buffers.
Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided.
Are Bio X Cell’s antibodies suitable for in vivo administration?
All of our products are suitable for in vivo use.
They are supplied in PBS without preservatives such as azide or protein stabilizers such as BSA.
The endotoxin levels are < 2EU/mg (InVivoMab) which qualifies them for most in vivo studies.
To meet the most stringent requirements we also offer antibodies with lower endotoxin levels: <1EU/mg (InVivoPlus).
What is the in vivo half-life of your antibody?
We do not have direct experimental data generated at Bio X Cell concerning the half-life of our antibodies in vivo.
Antibody half-life can be highly variable and influenced by a variety of factors including species, isotype, antigen distribution, antigen concentration and many others.
We recommend reading through the literature associated with the antibody you’re interested in to find published data in an experimental system similar to yours as a first step in estimating the half-life.
What dose or frequency of administration should I use to treat my mice?
The optimal dose and frequency of administration for any given experiment and antibody can vary greatly based on many details including the experimental system (i.e. mouse strain, disease model, etc.), the duration of the experiment, the target tissue, the antigen concentration, and much more.
For this reason, the optimal dose is best determined by reading through the product references associated with the antibody you’re interested in to find published data in an experimental system similar to yours.
Using this as a starting point you can then optimize the dose and frequency of administration experimentally.
Each of our products has an up-to-date reference list available on the products webpage.
What is the difference between Bio X Cell’s InVivoMab and InVivoPlus product lines?
Please see our InVivoPlus Difference page
What should I do about floaties, precipitates, or aggregates?
Environmental conditions such as temperature variations, freezing/thawing, shaking during transport or long term storage might lead to protein aggregation.
These aggregates are either settled in the conical tip of the vial or are floating freely in the suspension.
These aggregates can be removed by gentle shaking at room temperature; they can also be removed by filtration or centrifugation without significant loss.