RecombiMAb anti-mouse CD16/CD32

Clone Catalog # Category
2.4G2-CP025 CP025
USD 515 - USD 515

About RecombiMAb anti-mouse CD16/CD32

The 2.4G2-CP025 monoclonal antibody is a chimeric version of the original 2.4G2 antibody. The variable domain sequences are identical to the original 2.4G2 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The 2.4G2-CP025 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. 2.4G2-CP025 reacts specifically with mouse CD16 (FcγRIII) and CD32 (FcγRII). It has also been reported to react non-specifically via its Fc domain to FcγRI. CD16 and CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. These receptors bind to the Fc portion of antibody-antigen complexes and play a role in adaptive immune responses. The 2.4G2 antibody is commonly used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.

RecombiMAb anti-mouse CD16/CD32 Specifications

IsotypeMouse IgG2a, κ
ImmunogenBALB/c mouse macrophage cell line J774
Reported Applicationsin vitro Fc receptor blocking* in vivo Fc receptor blocking* *Reported for the original rat IgG2b 2.4G2 antibody
FormulationPBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation<5% Determined by SEC
Purity>95% Determined by SDS-PAGE
Sterility0.2 μm filtration
ProductionPurified from HEK293 cell supernatant in an animal-free facility
PurificationProtein G
Molecular Weight150 kDa
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.