RecombiMAb anti-mouse CD28

Clone Catalog # Category
D665-CP042 CP042
USD 515 - USD 515

About RecombiMAb anti-mouse CD28

The D665-CP042 monoclonal antibody is a chimeric version of the original D665 antibody. The variable domain sequences are identical to the original D665 but the constant region sequences have been switched from mouse IgG1 to mouse IgG2a. The D665-CP042 antibody contains no Fc mutations just as the original mouse IgG1 antibody does not. The D665-CP042 monoclonal antibody reacts with mouse CD28, a 45 kDa costimulatory receptor and a member of the Ig superfamily. CD28 is expressed by thymocytes, most peripheral T cells, and NK cells. CD28 is a receptor for CD80 (B7-1) and CD86 (B7-2). Signaling through CD28 induces IL-2 and IL-2 receptor expression and T cell proliferation. The D665 antibody is a CD28 superagonist and is most commonly used to induce the expansion of Treg cells in vivo in various mouse models of disease.

RecombiMAb anti-mouse CD28 Specifications

IsotypeMouse IgG2a, κ
ImmunogenA20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein
Reported Applicationsin vivo T cell stimulation/activation* in vitro T cell stimulation/activation* *Reported for the original mouse IgG1 D665 antibody
FormulationPBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation<5% Determined by SEC
Purity>95% Determined by SDS-PAGE
Sterility0.2 μm filtration
ProductionPurified from HEK293 cell supernatant in an animal-free facility
PurificationProtein G
Molecular Weight150 kDa
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.