RecombiMAb anti-mouse CD40L (CD154) (D265A)

Clone Catalog # Category
MR-1-CP032 CP032
USD 515 - USD 515

About RecombiMAb anti-mouse CD40L (CD154) (D265A)

The MR-1-CP032 monoclonal antibody is a chimeric version of the original MR-1 antibody. The variable domain sequences are identical to the original MR-1 but the constant region sequences have been switched from Armenian hamster IgG to mouse IgG1. The MR-1-CP032 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The MR-1-CP032 antibody reacts with mouse CD154 also known as CD40 ligand. CD154 exists as a 39 kDa accessory molecule and belongs to the TNF superfamily of cytokines. CD154 is primarily expressed on the surface of activated CD4+ T lymphocytes but can also be expressed by platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, CD8+ T lymphocytes as well as non-hematopoietic cells including smooth muscle cells, endothelial cells, and epithelial cells. CD154 signals through CD40 and is thought to play a key role in T and B lymphocyte costimulation. The MR-1 monoclonal antibody has been reported to inhibit in vitro activation of B lymphocytes by blocking the binding of CD154 with CD40 on T helper cells as well as inhibit the formation of germinal centers and disrupt antigen-specific T cell responses. Additionally, the MR-1 antibody blocks interactions of T cells and antigen-presenting cells in vitro and blocks the development of experimental autoimmune disease in vivo.

RecombiMAb anti-mouse CD40L (CD154) (D265A) Specifications

IsotypeMouse IgG1, κ
ImmunogenActivated mouse Th1 clone D1.6
Reported Applicationsin vivo blocking of CD40/CD40L signaling* in vitro blocking of CD40/CD40L signaling* Western blot* *Reported for the original Armenian hamster IgG MR-1 antibody
FormulationPBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation<5% Determined by SEC
Purity>95% Determined by SDS-PAGE
Sterility0.2 μm filtration
ProductionPurified from HEK293 cell supernatant in an animal-free facility
PurificationProtein G
Molecular Weight150 kDa
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.