RecombiMAb anti-mouse CSF1R (CD115)

Clone Catalog # Category
AFS98-CP131 CP131
USD 224 - USD 6087

About RecombiMAb anti-mouse CSF1R (CD115)

The AFS98-CP131 monoclonal antibody is a chimeric version of the original AFS98 antibody. The variable domain sequences are identical to the original AFS98 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The AFS98-CP131 antibody contains no Fc mutations just as the original rat IgG2a antibody does not. The AFS98-CP131 monoclonal antibody reacts with mouse colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115. CSF1R is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. In mice CSF1R is expressed by monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts. CSF1R is a receptor for CSF1 and CSF1 signaling through CSF1R regulates the proliferation and differentiation of cells in the monocytic lineage. The AFS98 antibody has been reported to deplete macrophages and block CSFR1 in vivo.

RecombiMAb anti-mouse CSF1R (CD115) Specifications

IsotypeMouse IgG2a, κ
ImmunogenNot available or unknown
Reported Applicationsin vivo macrophage depletion* in vitro CSF1R neutralization* in vivo monocyte depletion* Flow cytometry* Western blot* *Reported for the original rat IgG2a AFS98 antibody
FormulationPBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation<5% Determined by SEC
Purity>95% Determined by SDS-PAGE
Sterility0.2 μm filtration
ProductionPurified from CHO cell supernatant in an animal-free facility
PurificationProtein A
Molecular Weight150 kDa
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.