RecombiMAb anti-mouse CTLA-4 (CD152)

Clone Catalog # Category
9D9-CP006 CP006
USD 515 - USD 515

About RecombiMAb anti-mouse CTLA-4 (CD152)

The 9D9-CP006 monoclonal antibody is a chimeric version of the original 9D9 antibody. The variable domain sequences are identical to the original 9D9 but the constant region sequences have been switched from mouse IgG2b to mouse IgG1. 9D9-CP006 reacts with mouse CTLA-4 (cytotoxic T lymphocyte antigen-4) also known as CD152. CTLA-4 is a 33 kDa cell surface receptor encoded by the Ctla4 gene that belongs to the CD28 family of the Ig superfamily. CTLA-4 is expressed on activated T and B lymphocytes. CTLA-4 is structurally similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to the B7 family members B7-1 (CD80) and B7-2 (CD86). Upon ligand binding, CTLA-4 negatively regulates cell-mediated immune responses. CTLA-4 plays roles in induction and/or maintenance of immunological tolerance, thymocyte development, and regulation of protective immunity. The critical role of CTLA-4 in immune down-regulation has been demonstrated in CTLA-4 deficient mice, which succumb at 3-5 weeks of age due to the development of a lymphoproliferative disease. CTLA-4 is among a group of inhibitory receptors being explored as cancer treatment targets through immune checkpoint blockade. The 9D9 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding.

RecombiMAb anti-mouse CTLA-4 (CD152) Specifications

IsotypeMouse IgG1, κ
ImmunogenNot available or unknown
Reported Applicationsin vivo CTLA-4 neutralization* Western blot* *Reported for the original mouse IgG2b 9D9 antibody
FormulationPBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation<5% Determined by SEC
Purity>95% Determined by SDS-PAGE
Sterility0.2 μm filtration
ProductionPurified from HEK293 cell supernatant in an animal-free facility
PurificationProtein G
Molecular Weight150 kDa
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.