{"id":"830","post_author":null,"post_date":null,"post_date_gmt":null,"post_content":null,"post_title":"RecombiMAb anti-mouse LPAM-1 (Integrin \u03b14\u03b27)","post_excerpt":"The DATK32-CP069 monoclonal antibody is a recombinant, chimeric version of the original DATK32 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG2a, \u03ba to mouse IgG2a, \u03ba for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions\u2014including Fc receptor binding and complement activation\u2014and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models.  The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.\n\nThe DATK32 monoclonal antibody reacts with mouse LPAM-1 also known as integrin alpha 4 beta 7. The 130 kDa integrin \u03b27 chain associates with the 150 kDa integrin \u03b14 (CD49d) chain to form LPAM-1, a member of the Ig superfamily. LPAM-1 is expressed by peripheral lymphocytes, small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds VCAM-1 (CD106), MAdCAM-1, and fibronectin and facilitates lymphocyte adhesion and migration to the intestine and associated lymphoid tissues. The DATK32 antibody has been reported to block LPAM-1-mediated cell adhesion in vivo.","post_status":"publish","comment_status":null,"ping_status":null,"post_password":null,"post_name":"recombimab-anti-mouse-lpam-1-integrin-47-cp069","to_ping":null,"pinged":null,"post_modified":null,"post_modified_gmt":null,"post_content_filtered":null,"post_parent":null,"guid":null,"menu_order":null,"post_type":null,"post_mime_type":null,"comment_count":null,"filter":null,"sku":"CP069","path":"\/product\/recombimab-anti-mouse-lpam-1-integrin-47-cp069\/","image":"https:\/\/pneuma.bioxcell.com\/Media\/Products\/bioxcell-cp069-recombimab-anti-mouse-lpam-1-integrin-alpha4beta7-datk32-cp069.png","brand_id":null,"brand_name":null,"brand_slug":null,"brand_description":null,"isotype":"Mouse IgG2a,\u00a0\u03ba","immunogen":"TK1 cells","reported_applications":"in vivo Integrin \u03b14\u03b27 neutralization\nFlow cytometry\nFor information on in vivo applications, please contact technicalservice@bioxcell.com","aggregation":"<5%\nDetermined by SEC","formulation":"PBS, pH 7.0\nContains no stabilizers or preservatives","murine_pathogen_tests":"Mouse Norovirus: Negative\nMouse Parvovirus: Negative\nMouse Minute Virus: Negative\nMouse Hepatitis Virus: Negative\nReovirus Screen: Negative\nLymphocytic Choriomeningitis virus: Negative\nLactate Dehydrogenase-Elevating Virus: Negative\nMouse Rotavirus: Negative\nTheiler's Murine Encephalomyelitis: Negative\nEctromelia\/Mousepox Virus: Negative\nHantavirus: Negative\nPolyoma Virus: Negative\nMouse Adenovirus: Negative\nSendai Virus: Negative\nMycoplasma Pulmonis: Negative\nPneumonia Virus of Mice: Negative\nMouse Cytomegalovirus: Negative\nK Virus: Negative","human_pathogen_tests":"","toxicity_test_results":null,"endotoxin":"<ul><li>\u22640.5EU\/mg (\u22640.0005EU\/\u03bcg)<\/li><li>Determined by LAL gel clotting assay<\/li><\/ul>","purity":"<ul><li>\u226595%<\/li><li>Determined by SDS-PAGE<\/li><\/ul>","sterility":"0.2 \u03bcm filtration","production":"Purified from HEK293 cell supernatant in an animal-free facility","purification":"Protein G","composition":null,"volume":null,"concentration":null,"molecular_weight":"150 kDa","storage":"The antibody solution should be stored at the stock concentration at 4\u00b0C. Do not freeze.","rrid":"","recommended_isotype_control":"<a href=\"\/product\/\">RecombiMAb mouse IgG2a isotype control, unknown specificity<\/a>","recommended_dilution_buffer":"<a href=\"\/product\/\">InVivoPure pH 7.0 Dilution Buffer<\/a>","clone":"DATK32-CP069","technical_data_sheet":"https:\/\/pneuma.bioxcell.com\/api\/v1\/products\/certificate-of-analysis\/CP069\/0\/0\/0?apikey=23D50188E6F74755AFFBF95BD41B140B","western_blot_image":null,"new_product_image":null,"product_references":null,"display_pubmed_logo":null,"modified":"2026-03-21 02:10:05","active":"Y"}

RecombiMAb anti-mouse LPAM-1 (Integrin α4β7)

Clone	Catalog #	Category
DATK32-CP069	CP069

USD 541 - USD 7325

Technical Data Sheet

SDS Sheet

About RecombiMAb anti-mouse LPAM-1 (Integrin α4β7)

The DATK32-CP069 monoclonal antibody is a recombinant, chimeric version of the original DATK32 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG2a, κ to mouse IgG2a, κ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The DATK32 monoclonal antibody reacts with mouse LPAM-1 also known as integrin alpha 4 beta 7. The 130 kDa integrin β7 chain associates with the 150 kDa integrin α4 (CD49d) chain to form LPAM-1, a member of the Ig superfamily. LPAM-1 is expressed by peripheral lymphocytes, small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds VCAM-1 (CD106), MAdCAM-1, and fibronectin and facilitates lymphocyte adhesion and migration to the intestine and associated lymphoid tissues. The DATK32 antibody has been reported to block LPAM-1-mediated cell adhesion in vivo.

RecombiMAb anti-mouse LPAM-1 (Integrin α4β7) Specifications

Isotype	Mouse IgG2a, κ
Recommended Isotype Control(s)	RecombiMAb mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Immunogen	TK1 cells
Reported Applications	in vivo Integrin α4β7 neutralization Flow cytometry For information on in vivo applications, please contact technicalservice@bioxcell.com
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay
Aggregation	<5% Determined by SEC
Purity	≥95% Determined by SDS-PAGE
Sterility	0.2 μm filtration
Production	Purified from HEK293 cell supernatant in an animal-free facility
Purification	Protein G
Molecular Weight	150 kDa
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.