About RecombiMAb anti-mouse Ly6G/Ly6C (Gr-1) The RB6-8C5-CP135 monoclonal antibody is a chimeric version of the original RB6-8C5 antibody. The variable domain sequences are identical to the original RB6-8C5 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The RB6-8C5-CP135 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The RB6-8C5-CP135 monoclonal antibody reacts strongly with mouse Ly6G and weakly with mouse Ly6C previously referred to as GR-1. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes macrophages granulocytes and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations.The RB6-8C5 antibody has been shown to inhibit the binding of the 1A8 antibody. The 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C. RecombiMAb anti-mouse Ly6G/Ly6C (Gr-1) Specifications IsotypeMouse IgG2a, κ Recommended Isotype Control(s)InVivoPlus mouse IgG2a isotype control, unknown specificity Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer ImmunogenMouse granulocytes Reported Applicationsin vivo depletion of Gr-1+ myeloid cells* Flow cytometry* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* *Reported for the original rat IgG2b RB6-8C5 antibody FormulationPBS, pH 7.0 Contains no stabilizers or preservatives Endotoxin<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay Aggregation<5% Determined by SEC Purity>95% Determined by SDS-PAGE Sterility0.2 μm filtration ProductionPurified from CHO cell supernatant in an animal-free facility PurificationProtein G Molecular Weight150 kDa StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
