{"id":"512","post_author":null,"post_date":null,"post_date_gmt":null,"post_content":null,"post_title":"RecombiMAb anti-mouse Ly6G\/Ly6C (Gr-1)","post_excerpt":"The RB6-8C5-CP135 monoclonal antibody is a chimeric version of the original RB6-8C5 antibody. The variable domain sequences are identical to the original RB6-8C5 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The RB6-8C5-CP135 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The RB6-8C5-CP135 monoclonal antibody reacts strongly with mouse Ly6G and weakly with mouse Ly6C previously referred to as GR-1. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes macrophages granulocytes and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations.The RB6-8C5 antibody has been shown to inhibit the binding of the 1A8 antibody. The 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.\n","post_status":"publish","comment_status":null,"ping_status":null,"post_password":null,"post_name":"recombimab-anti-mouse-ly6gly6c-gr-1-cp135","to_ping":null,"pinged":null,"post_modified":null,"post_modified_gmt":null,"post_content_filtered":null,"post_parent":null,"guid":null,"menu_order":null,"post_type":null,"post_mime_type":null,"comment_count":null,"filter":null,"sku":"CP135","path":"\/product\/recombimab-anti-mouse-ly6gly6c-gr-1-cp135\/","image":"https:\/\/pneuma.bioxcell.com\/Media\/Products\/bioxcell-cp135-recombimab-anti-mouse-ly6g-ly6c-gr-1-rb6-8c5-cp135.png","brand_id":null,"brand_name":null,"brand_slug":null,"brand_description":null,"isotype":"Mouse IgG2a, \u03ba","immunogen":"Mouse granulocytes","reported_applications":"in vivo depletion of Gr-1+ myeloid cells*\nFlow cytometry*\nImmunohistochemistry (paraffin)*\nImmunohistochemistry (frozen)*\n*Reported for the original rat IgG2b RB6-8C5 antibody","aggregation":"<5%\nDetermined by SEC","formulation":"PBS, pH 7.0\nContains no stabilizers or preservatives","murine_pathogen_tests":"Mouse Norovirus: Negative\nMouse Parvovirus: Negative\nMouse Minute Virus: Negative\nMouse Hepatitis Virus: Negative\nReovirus Screen: Negative\nLymphocytic Choriomeningitis virus: Negative\nLactate Dehydrogenase-Elevating Virus: Negative\nMouse Rotavirus: Negative\nTheiler's Murine Encephalomyelitis: Negative\nEctromelia\/Mousepox Virus: Negative\nHantavirus: Negative\nPolyoma Virus: Negative\nMouse Adenovirus: Negative\nSendai Virus: Negative\nMycoplasma Pulmonis: Negative\nPneumonia Virus of Mice: Negative\nMouse Cytomegalovirus: Negative\nK Virus: Negative","human_pathogen_tests":"","toxicity_test_results":null,"endotoxin":"<ul><li>\u22640.5EU\/mg (\u22640.0005EU\/\u03bcg)<\/li><li>Determined by LAL gel clotting assay<\/li><\/ul>","purity":"<ul><li>\u226595%<\/li><li>Determined by SDS-PAGE<\/li><\/ul>","sterility":"0.2 \u03bcm filtration","production":"Purified from CHO cell supernatant in an animal-free facility","purification":"Protein G","composition":null,"volume":null,"concentration":null,"molecular_weight":"150 kDa","storage":"The antibody solution should be stored at the stock concentration at 4\u00b0C. Do not freeze.","rrid":"","recommended_isotype_control":"<a href=\"\/product\/\">InVivoPlus mouse IgG2a isotype control, unknown specificity<\/a>","recommended_dilution_buffer":"<a href=\"\/product\/\">InVivoPure pH 7.0 Dilution Buffer<\/a>","clone":"RB6-8C5-CP135","technical_data_sheet":"https:\/\/pneuma.bioxcell.com\/api\/v1\/products\/certificate-of-analysis\/CP135\/0\/0\/0?apikey=23D50188E6F74755AFFBF95BD41B140B","western_blot_image":null,"new_product_image":null,"product_references":null,"display_pubmed_logo":null,"modified":"2026-03-21 02:10:05","active":"Y"}

RecombiMAb anti-mouse Ly6G/Ly6C (Gr-1)

Clone	Catalog #	Category
RB6-8C5-CP135	CP135

USD 235 - USD 6391

Technical Data Sheet

SDS Sheet

About RecombiMAb anti-mouse Ly6G/Ly6C (Gr-1)

The RB6-8C5-CP135 monoclonal antibody is a chimeric version of the original RB6-8C5 antibody. The variable domain sequences are identical to the original RB6-8C5 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The RB6-8C5-CP135 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The RB6-8C5-CP135 monoclonal antibody reacts strongly with mouse Ly6G and weakly with mouse Ly6C previously referred to as GR-1. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes macrophages granulocytes and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations.The RB6-8C5 antibody has been shown to inhibit the binding of the 1A8 antibody. The 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.

RecombiMAb anti-mouse Ly6G/Ly6C (Gr-1) Specifications

Isotype	Mouse IgG2a, κ
Recommended Isotype Control(s)	InVivoPlus mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Immunogen	Mouse granulocytes
Reported Applications	in vivo depletion of Gr-1+ myeloid cells* Flow cytometry* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* *Reported for the original rat IgG2b RB6-8C5 antibody
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay
Aggregation	<5% Determined by SEC
Purity	≥95% Determined by SDS-PAGE
Sterility	0.2 μm filtration
Production	Purified from CHO cell supernatant in an animal-free facility
Purification	Protein G
Molecular Weight	150 kDa
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.